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Home/ Questions/Q 150768
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Madhu Mati
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Madhu Mati
Asked: October 12, 20212021-10-12T10:19:57+00:00 2021-10-12T10:19:57+00:00In: Class 12 Biology

You have chosen a plasmid as vector for cloning your gene. However this vector plasmid lacks a selectable marker. How would it affect your experiment?

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      Vansh Varma
      2021-10-14T04:49:54+00:00Added an answer on October 14, 2021 at 4:49 am

      In a gene cloning experiment, first a recombinant DNA molecule is constructed, where the gene of interest is ligated to the vector, [The step would not be affected] and introduced inside the host cell (transformation). Since, not all the cells get transformed with the recombinant / plasmid DNA, in the absence of selectable marker, it will be difficult to distinguish between transformants and non-transformant, because role of selectable marker is in the selection of transformants.

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