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Home/ Questions/Q 150909
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Ragni
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Ragni
Asked: October 12, 20212021-10-12T10:20:03+00:00 2021-10-12T10:20:03+00:00In: Class 12 Biology

A mixture of fragmented DNA was electrophoresed in agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?

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      Vansh Varma
      2021-10-14T04:49:58+00:00Added an answer on October 14, 2021 at 4:49 am

      The reasons are as follows:
      (i) DNA sample that was loaded on the gel may have got contaminated with nuclease (exo-or endo-or both) and completely degraded.
      (ii) Electrodes were put in opposite orientation in the gel assembly that is anode towards the wells (where DNA sample is loaded). Since DNA molecules are negatively charged, they move towards
      anode and hence move out of the gel instead of moving into the matrix of gel.
      iii) Ethidium bromide was not added at all or was not added in sufficient concentration and so DNA was not visible.

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