Class 12 Biology Extra Questions Answers
Class 12th Biology NCERT Based Questions
Class 12 Science Biology Terminal Exams Solutions
12th Biology Science Session 2022-23
You have chosen a plasmid as vector for cloning your gene. However this vector plasmid lacks a selectable marker. How would it affect your experiment?
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In a gene cloning experiment, first a recombinant DNA molecule is constructed, where the gene of interest is ligated to the vector, [The step would not be affected] and introduced inside the host cell (transformation). Since, not all the cells get transformed with the recombinant / plasmid DNA, in the absence of selectable marker, it will be difficult to distinguish between transformants and non-transformant, because role of selectable marker is in the selection of transformants.