The branch of botanical science concerned with the separation and combination of living matter is called tissue culture. Tissue culture, also known as plant tissue culture or micropropagation, involves growing plant tissues, cells, or organs in a controlled, sterile environment. This technique allows scientists to study plant development, genetics, and physiology in detail. Tissue culture is used for the propagation of plants, enabling the rapid production of large numbers of genetically identical plants from a small piece of plant tissue. It is also employed in the conservation of rare and endangered plant species by preserving their genetic material. Additionally, tissue culture plays a significant role in plant breeding, biotechnology, and research on plant diseases. This method has revolutionized horticulture and agriculture by providing a means to produce disease-free and uniform plants efficiently.