Oxidative phosphorylation is a process in which electrons are transferred from electron donors to oxygen, which acts as electron acceptor. The oxidation-reduction reactions are involved in the formation of proton gradient. The main role in oxidative phosphorylation is played by the enzyme ATP synthase (complex V). This enzyme complex consists of F₀ and F₁ components. The F1 headpiece is a peripheral membrane protein complex and contains the site for ATP synthesis from ADP and inorganic phosphate. F0 component is a part of membrane protein complex, which acts as a channel for crossing of the protons from inner mitochondrial membrane to the mitochondrial matrix. For every two protons passing through F₀ –F₁ complex, synthesis of one ATP molecule takes place.

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