Agrobacterium tumefaciens harbours a mega plasmid called Ti-plasmid. This has a T-DNA region flanked by left border and right border sequence. The T-DNA gets transferred and integrates with the host plant DNA. This property of Ti-plasmid has been exploited for cloning of gene of interest and stably integrating them in the plant genese. Therefore, by using Ti-plasmid or its derivatives, recombinant plant cells with desired genes of interest stably integrated in the plant genome has been successfully produced.