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Asked: October 12, 2021In: Class 12 Biology

The restriction enzymes that are used in construction of recombinant DNA molecule are endonucleases which cut the DNA at ‘specificrecognition sequence’. What would be the disadvantage if they would not cut the DNA at specific-recognition sequence?

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Asked: October 12, 2021In: Class 12 Biology

You have created a recombinant DNA molecule by ligating a gene to a plasmid vector. By mistake, your friend adds exonuclease enzyme to the tube containing the recombinant DNA. How will your experiment get affected as you plan to go for transformation now?

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