Class 12 Science Biology Important Questions
Class XII Science Biology Extra Questions
Class 12th Biology Sample paper Questions
Class 12 Biology Extra Questions Answers
Would you like to choose an exonuclease enzyme while producing a recombinant DNA molecule?
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No, as exonuclease acts on the free ends of linear DNA molecule. Therefore, instead of producing DNA fragments with sticky ends, it will shorten or completely degrade the DNA fragment containing the gene of interest, and the circular plasmid (vector) will not get cut as it lacks free ends.