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Name the five key tools for accomplishing the tasks of recombinant DNA technology. Also mention the functions of each tool.

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  1. i. Restriction endonucleases: for cutting the desired DNA at desired places
    ii. Gel electrophoresis: for separating the desired DNA fragments
    iii. Ligase enzyme: for creating recombinant DNA molecule.
    iv. DNA delivery system: like electroporation, microinjection, gene gun method.
    v. Competant host (usually bacteria / yeast): to take up recombinant DNA.

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