A nucleoside is formed by attaching a base to the 1’ position of a sugar. Nucleotides are created when a nucleoside links to phosphoric acid at the 5’-position of the sugar, and nucleotides join through phosphodiester linkages.
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A nucleoside is a molecule composed of a nitrogenous base (either adenine, guanine, cytosine, thymine, or uracil) and a sugar (ribose or deoxyribose) but lacks the phosphate group found in nucleotides. Nucleotides are formed when a phosphate group is attached to the 5′ carbon of the sugar in a nucleoside through a phosphodiester bond. The process involves the condensation of the phosphate group with the hydroxyl group on the 5′ carbon of the sugar. Nucleotides are the building blocks of nucleic acids, such as DNA and RNA, with the sequence of nucleotides encoding genetic information.