The reasons are as follows: (i) DNA sample that was loaded on the gel may have got contaminated with nuclease (exo-or endo-or both) and completely degraded. (ii) Electrodes were put in opposite orientation in the gel assembly that is anode towards the wells (where DNA sample is loaded). Since DNA moRead more
The reasons are as follows:
(i) DNA sample that was loaded on the gel may have got contaminated with nuclease (exo-or endo-or both) and completely degraded.
(ii) Electrodes were put in opposite orientation in the gel assembly that is anode towards the wells (where DNA sample is loaded). Since DNA molecules are negatively charged, they move towards
anode and hence move out of the gel instead of moving into the matrix of gel.
iii) Ethidium bromide was not added at all or was not added in sufficient concentration and so DNA was not visible.
In a gene cloning experiment, first a recombinant DNA molecule is constructed, where the gene of interest is ligated to the vector, [The step would not be affected] and introduced inside the host cell (transformation). Since, not all the cells get transformed with the recombinant / plasmid DNA, in tRead more
In a gene cloning experiment, first a recombinant DNA molecule is constructed, where the gene of interest is ligated to the vector, [The step would not be affected] and introduced inside the host cell (transformation). Since, not all the cells get transformed with the recombinant / plasmid DNA, in the absence of selectable marker, it will be difficult to distinguish between transformants and non-transformant, because role of selectable marker is in the selection of transformants.
A compound called Ethidium Bromide stains DNA, which on irradiating with Ultra Violet, fluoresce gives orange light. Hence, DNA fragments appear as orange band in the presence of Ethidium B romide and UV.
A compound called Ethidium Bromide stains DNA, which on irradiating with Ultra Violet, fluoresce gives orange light. Hence, DNA fragments appear as orange band in the presence of Ethidium B romide and UV.
Under which of the following type of resource can tidal energy be put?
(B) Human-made
(B) Human-made
See lessWe can obtain per capita income of a country by calculating:
(B) by dividing the national income by the total population of a country
(B) by dividing the national income by the total population of a country
See lessHuman Development Index compares countries based on which of the following levels of the People?
(d) All the above
(d) All the above
See lessWhich one of the following impurities is easiest to remove from wastewater?
d. Suspended solids
d. Suspended solids
See lessWhich of the following statements is correct?
b. African catfish is not a threat to indigenous catfishes.
b. African catfish is not a threat to indigenous catfishes.
See lessWhat is common to Lantana, Eichhornia and African catfish?
d. All the species are neither threatened nor indigenous species of India.
d. All the species are neither threatened nor indigenous species of India.
See lessAmongst the animal groups given below, which one has the highest percentage of endangered species?
c. Amphibians
c. Amphibians
See lessA mixture of fragmented DNA was electrophoresed in agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?
The reasons are as follows: (i) DNA sample that was loaded on the gel may have got contaminated with nuclease (exo-or endo-or both) and completely degraded. (ii) Electrodes were put in opposite orientation in the gel assembly that is anode towards the wells (where DNA sample is loaded). Since DNA moRead more
The reasons are as follows:
See less(i) DNA sample that was loaded on the gel may have got contaminated with nuclease (exo-or endo-or both) and completely degraded.
(ii) Electrodes were put in opposite orientation in the gel assembly that is anode towards the wells (where DNA sample is loaded). Since DNA molecules are negatively charged, they move towards
anode and hence move out of the gel instead of moving into the matrix of gel.
iii) Ethidium bromide was not added at all or was not added in sufficient concentration and so DNA was not visible.
You have chosen a plasmid as vector for cloning your gene. However this vector plasmid lacks a selectable marker. How would it affect your experiment?
In a gene cloning experiment, first a recombinant DNA molecule is constructed, where the gene of interest is ligated to the vector, [The step would not be affected] and introduced inside the host cell (transformation). Since, not all the cells get transformed with the recombinant / plasmid DNA, in tRead more
In a gene cloning experiment, first a recombinant DNA molecule is constructed, where the gene of interest is ligated to the vector, [The step would not be affected] and introduced inside the host cell (transformation). Since, not all the cells get transformed with the recombinant / plasmid DNA, in the absence of selectable marker, it will be difficult to distinguish between transformants and non-transformant, because role of selectable marker is in the selection of transformants.
See lessHow do you visualise DNA on an agarose gel?
A compound called Ethidium Bromide stains DNA, which on irradiating with Ultra Violet, fluoresce gives orange light. Hence, DNA fragments appear as orange band in the presence of Ethidium B romide and UV.
A compound called Ethidium Bromide stains DNA, which on irradiating with Ultra Violet, fluoresce gives orange light. Hence, DNA fragments appear as orange band in the presence of Ethidium B romide and UV.
See less