Role of proteases is to degrade the proteins present inside a cell (from which DNA is being isolated). If the proteins are not removed from DNA preparation then they could interfere with any downstream treatment of DNA (such action of restriction endonuclease, DNA ligase etc).
Role of proteases is to degrade the proteins present inside a cell (from which DNA is being isolated). If the proteins are not removed from DNA preparation then they could interfere with any downstream treatment of DNA (such action of restriction endonuclease, DNA ligase etc).
It the restriction enzymes have more than one recognition site in a vector, than the vector itself will get fragmented on treatment with the restriction enzyme.
It the restriction enzymes have more than one recognition site in a vector, than the vector itself will get fragmented on treatment with the restriction enzyme.
No, as exonuclease acts on the free ends of linear DNA molecule. Therefore, instead of producing DNA fragments with sticky ends, it will shorten or completely degrade the DNA fragment containing the gene of interest, and the circular plasmid (vector) will not get cut as it lacks free ends.
No, as exonuclease acts on the free ends of linear DNA molecule. Therefore, instead of producing DNA fragments with sticky ends, it will shorten or completely degrade the DNA fragment containing the gene of interest, and the circular plasmid (vector) will not get cut as it lacks free ends.
The digestion of food starts in “which labelled”?
[D] Mouth Label A represents mouth. The digestion of food starts in mouth.
[D] Mouth
See lessLabel A represents mouth. The digestion of food starts in mouth.
If the object is kept at 40 cm, the position and nature of image formed will be:
[B] Real and inverted image will be formed 40 cm in front of the mirror of same size as object.
[B] Real and inverted image will be formed 40 cm in front of the mirror of same size as object.
See lessWhat is the significance of adding proteases at the time of isolation of genetic material (DNA).
Role of proteases is to degrade the proteins present inside a cell (from which DNA is being isolated). If the proteins are not removed from DNA preparation then they could interfere with any downstream treatment of DNA (such action of restriction endonuclease, DNA ligase etc).
Role of proteases is to degrade the proteins present inside a cell (from which DNA is being isolated). If the proteins are not removed from DNA preparation then they could interfere with any downstream treatment of DNA (such action of restriction endonuclease, DNA ligase etc).
See lessRestriction enzymes present in the cloning site of a vector should not have more than one recognition site. Comment.
It the restriction enzymes have more than one recognition site in a vector, than the vector itself will get fragmented on treatment with the restriction enzyme.
It the restriction enzymes have more than one recognition site in a vector, than the vector itself will get fragmented on treatment with the restriction enzyme.
See lessWould you like to choose an exonuclease enzyme while producing a recombinant DNA molecule?
No, as exonuclease acts on the free ends of linear DNA molecule. Therefore, instead of producing DNA fragments with sticky ends, it will shorten or completely degrade the DNA fragment containing the gene of interest, and the circular plasmid (vector) will not get cut as it lacks free ends.
No, as exonuclease acts on the free ends of linear DNA molecule. Therefore, instead of producing DNA fragments with sticky ends, it will shorten or completely degrade the DNA fragment containing the gene of interest, and the circular plasmid (vector) will not get cut as it lacks free ends.
See less