Benzoylation is a specific acylation reaction where amines react with benzoyl chloride (C₆H₅COCl). Pyridine, a base stronger than the amine, is used in acylation reactions to remove HCl formed during the reaction and shift the equilibrium towards the right.

Aliphatic and aromatic primary and secondary amines undergo acylation, a nucleophilic substitution reaction with acid chlorides, anhydrides, and esters. The products of acylation reactions are known as amides.

Amines undergo alkylation when they react with alkyl halides, resulting in the substitution of hydrogen atoms with alkyl groups.

Electron-releasing groups like –OCH₃, –CH₃ increase the basic strength of substituted aniline, while electron-withdrawing groups like –NO₂, –SO₃H, –COOH, –X decrease it. This observation highlights the role of substituents in influencing the basic nature of aromatic amines.

Aniline, with five resonating structures, is more stable than the anilinium ion, which has only two resonating structures (kekule). The greater stability of aniline contributes to its lower proton acceptability or basic nature compared to ammonia.