NCERT Important Questions Class 10 Science
CBSE 2022-2023.
NCERT Book Extra Questions for Session 2022-2023
CBSE Board and UP Board Others state Board
NCERT book extra.
Write correct sequence of four steps of method for the preparation of temporary mount of a stained leaf peel.
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Four steps for preparing stained mount of leaf peel are:
(i) Take out the leaf peel from lower surface of the leaf.
(ii) Place it on a slide on a drop of ‘water and safranin stain.
(iii) Absorb excess stain using blotting paper and add a drop of glycerine.
(iv) Put coverslip so as to avoid any air bubble and observe under the microscope.
1. Take a healthy leaf from the potted plant.
2. Remove a part of the peel from the lower surface of the leaf. You can do this by folding the leaf and gently pulling the peel apart using forceps. Keeps the peel in a watch glass containing
water.
3. Put a few drops of safranin stain in a watch glass.
4. After 2-3 minutes take out the peel and place it on a clean glass slide.
5. Put a drop of glycerin over the peel and place a clean cover lip gently over it with the help of a needle.
6. Remove the excess stain and glycerin with the help of blotting paper.
7. Observe the slide under magnifications of the compound microscope.
Best answer