The leaves of C4 plants have a special anatomy called Kranz anatomy. This makes them different from C3 plants. Special cells, known as bundle-sheath cells, surround the vascular bundles. These cells have a large number of chloroplasts. They are thick-walled and have no intercellular spaces. They are also impervious to gaseous exchange. All these anatomical features help prevent photorespiration in C₄ plants, thereby increasing their ability to photosynthesise.

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